Anti-Rabbit IgG Donor Beads

The Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer between donor beads and acceptor beads at close proximity, resulting in luminescence.

Donor beads recognize Protein 1 (Tag1 label), while Acceptor beads recognize Protein 2 (Tag2 label). When Protein 1 binds to Protein 2, the distance between the beads becomes less than 200nm. Upon excitation at 680nm, the donor beads generate singlet oxygen, which diffuses to the Acceptor beads. The Acceptor beads then undergo a redox reaction, emitting light at 615nm. The signal intensity is directly proportional to the strength of the protein interaction.

This product features a simple operation process, requires no washing, and offers high speed and sensitivity. It is capable of detecting weak interactions.

1. This experiment is light-sensitive. Perform all operations (preparation, pipetting, and incubation) under green light (illuminance below 100 LUX) to avoid light exposure.

2. This product is compatible with multifunctional microplate readers equipped with an Alpha detection module.

3. Vortex thoroughly before use. Alternatively, briefly centrifuge (2000×g, 5–10 seconds) to ensure complete sample retrieval.

4. Use the accompanying dilution buffer provided by our company for reagent preparation and sample dilution. If additional components are required, they may be directly added to this buffer.

5. To ensure comparability of experimental data across different batches, strictly control incubation temperature and duration.

6. Avoid bubble formation during pipetting.