SKU: 89684198360

UniOne® TR-FRET Human CRBN Ligand Screening Assay Kit

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Description

UniOne® TR-FRET Human CRBN Ligand Screening Assay KitProduct Specification Host Human Stability & Storage 80C Background The Human Cereblon (CRBN) Protein Ligand Binding Assay Kit utilizes homogeneous time resolved fluorescence (TR FRET) technology and can be used for the binding detection and screening of CRBN inhibitors or ligands. It enables high throughput screening for rapid detection of CRBN inhibitors or ligands, with the basic principle illustrated in the figure below. The Eu labeled detection

Product Specification


Host Human
Stability & Storage

-80°C

Background

The Human Cereblon (CRBN) Protein Ligand Binding Assay Kit utilizes homogeneous time-resolved fluorescence (TR-FRET) technology and can be used for the binding detection and screening of CRBN inhibitors or ligands. It enables high-throughput screening for rapid detection of CRBN inhibitors or ligands, with the basic principle illustrated in the figure below. The Eu-labeled detection reagent (Anti-Tag1-Eu) specifically recognizes and binds to the Tag1-CRBN/DDB1 protein, while the receptor-labeled thalidomide (Thalidomide-Ac) binds to CRBN, bringing Eu and Ac into close proximity. Under excitation by an external light source, energy resonance transfer occurs between the donor and acceptor, and the signal intensity at 665 nm can be measured to determine the binding of CRBN/DDB1 to Thalidomide-Ac. The addition of CRBN ligands or PROTACs to the reaction system competitively binds to CRBN/DDB1, reducing the signal. As the concentration of ligands or PROTACs increases, the signal decreases. This homogeneous assay is simple to perform and does not require washing steps.


Components

Component

Concentration

Volume(100T)

Volume(500T)

Volume(2500T)

Volume(10000T)

Storage Condition

Thalidomide

10 mM in DMSO

5 μL

5 μL

25 μL

100 μL

-80℃

Tag1-CRBN/DDB1

100×

5μL

25μL

125μL

500 μL

-80℃

Thalidomide-Ac

100×

5μL

25μL

125μL

500 μL

-80℃

Anti-Tag1-Eu

50×

10μL

50μL

250 μL

1000 μL

-80℃

Detection Buffer

10×

250μL

1.2mL

6mL

21 mL

-80℃

*Reagents should be protected from repeated freeze-thaw cycles. It is recommended to aliquot after the first thaw and store according to the storage conditions. The shelf life is one year.
*Thaw reagents on ice before use and equilibrate to room temperature.
*Prepare all reagent working solutions freshly before use.

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Exchange/Return Notes
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SKU: 89684198360

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M
Charlottesville, US
★★★★★ 5
Function and style
Size: 11 Women/10 Men, Color: Black Blizzard Wp
Ideal boot for the winter, snow, mud, etc. Protect your feet beautifully, stylishly. Tip: Wear with two socks to help break in for the first couple weeks. If you are a half size, round up. This is the model without laces, makes for easy slip on / slip off for those pesky people who insist on having you take off your shoes at their house! Ha ha, you know who you are.
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Reviewed in the United States on April 13, 2026
M
Verified Purchase
Michael
Draper, US
★★★★★ 4
Size up for a little bit of heel slip
Size: 9 Women/8 Men, Color: Black Nappa
It’s still a doc and the air sole with bottom out kinda quick but it’s comfortable at first. Size up for a little bit of heel slip so you don’t get heel blisters but not too big of a size jump. Think of it kind of like a cowboy boot size
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Reviewed in the United States on December 30, 2025
C
Verified Purchase
Chris and Dawn Grimes
Natrona Heights, US
★★★★★ 5
Comfy, don't require "breaking in"
Size: 8 Women/7 Men, Color: Dark Brown Valor Wp
Great fit, and both my pairs of Chelsea boots required way less breaking in than other Dr. Martens I own. Hold up to frequent use and I love the fleece lining for colder days.
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Reviewed in the United States on December 29, 2025
J
Verified Purchase
Jason D. Covell
Belleville, US
★★★★★ 5
Excellent boots
Size: 11 Women/10 Men, Color: Black Nappa
Excellent boots
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Reviewed in the United States on May 26, 2026
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Verified Purchase
Juan A. Flores
Waukegan, US
★★★★★ 5
Great set of boots
Size: 11 Women/10 Men, Color: Black Nappa
Overall great product, I’m very happy with the purchase.
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Reviewed in the United States on April 18, 2026

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